黄绿木霉菌代谢产物对大豆菌核病核盘菌的抑菌能力研究

Study on the Restrain Sclerotinia sclerotiorum by Trichoderma aureoviride Metabolite

利用室内实验的方法,研究了黄绿木霉对大豆菌核病菌的抑菌能力。黄绿木霉在与核盘菌(Sclerotiniasclerotiorum)对峙培养过程中,5～7d即可将菌核病菌完全覆盖,在形成的菌核上会有黄绿木霉孢子出现,扫描电镜中观察到核盘菌的菌丝体发生变形;其代谢产物在经121℃处理25min后,抑菌作用仍可高达100%;在对黄绿木霉发酵过程中,利用PD培养基即可达到有效的控制大豆菌核病菌菌丝生长的目的,而且利用PD培养基发酵3～4d的黄绿木霉代谢产物抑菌效果最好,抑菌中浓度最低,发酵时间过长与过短,均不利于对病原菌的抑制。经黄绿木霉代谢产物处理的菌核菌电导率与呼吸强度均发生变化,在光学显微镜下观察到菌核菌菌体发生变化,且处理后的菌核菌致病力明显降低。

By means of the trails in doors, the ability of restrain S. sclerotiorum by T. aureoviride was studied. When the metabolite was treated at 121 centigrade dgree for 25 minutes, the ability of restrain mycelium growth was reserved,and while diluted one diplosis, the restrain ability was 100 %. In order to control mycelium growth effectively, the optimal medium was PD and the optimal time for ferment was three to four days. The electric conductivity and respiratory intensity of S. sclerotiorum mycelium treated by T. aureoviride metabolite were also changed, and the ability of inducing disease also decreased.