摘要
考察葛根素对胃蛋白酶的抑制作用,并通过紫外光谱法和荧光光谱法研究葛根素与胃蛋白酶的结合方式。以1/v对抑制剂量用Dixon作图法得出ki值为0.51×103mol/L,抑制剂类型为非竞争性抑制。葛根素与胃蛋白酶分子结合形成稳定缔合物,从而改变后者分子构象,使胃蛋白酶的紫外吸收差谱迅速增强,特征荧光峰产生静态淬灭。
In addition to studying the linking pattem between puterrin and pepsin by using ultraviolet and fluorescent spectrometry, the inhibition of puterrin over pepsin was investigated in tiffs research, ki（0.51×10^-3 mol/L） was educed by Dixon graphieal chart with 1/v versus amount of inhibitor. The result shows that inhibitor is a noncompetitive one. The bondage between puterrin and pepsin forms a stable complex compound, which alters the molecular configuration of the latter, enhances UV absorption of pepsin, and results in a static quenching of puterrin to pepsin.
出处
《天然产物研究与开发》
CAS
CSCD
2005年第6期704-707,共4页
Natural Product Research and Development
关键词
胃蛋白酶
葛根素
抑制作用
紫外光谱
荧光光谱
pepsin
puterrin
prohibitive function
ultraviolet spectra
fluorescent spectra
