小麦中提取的α-淀粉酶抑制剂调节血脂及抗动脉粥样硬化的实验研究

Lipid-regulating and Anti-atherosclerosis Effects of α-Amylase Inhibitor Extracted from Wheat

目的:研究小麦中提取的α-淀粉酶抑制剂(简称-αAI)调节血脂及抗动脉粥样硬化的作用。方法:预防性给药时,给高脂饲料造模的同时连续给药9周,于第63天测定大鼠血清TC、TG、HDL-C、LDL-C、MDA、SOD及肝脏TC、TG含量,同时观察其对大鼠血液流变学及动脉、肝脏病变的影响。治疗性给药时,给高脂饲料造模成功后连续给药3周,测定大鼠血清TC、TG、HDL-C、LDL-C及肝脏TC、TG含量。结果:预防性给药时,中、高剂量-αAI能显著降低血清TC、TG、LDL-C及肝脏TC、TG含量,显著升高HDL-C,还可以使MDA含量显著降低,SOD活性显著升高,同时可以改善血液流变学,缓解动脉及肝脏病变情况。治疗性给药时,高剂量-αAI能显著降低血清TC、TG、LDL-C及肝脏TC、TG含量,显著升高HDL-C。结论:-αAI能调节血脂平衡,预防高脂血症及动脉粥样硬化的生成,对已形成的高脂血症也有一定的治疗效果。

AIM： To study the lipid-regulating and anti-atherosclerosis effects of α-amylase inhibitor. METHODS： In the prevention study, the rats were raised with high fat diet for 9 weeks and co-administered with various doses of α-amylase inhibitor. The concentration of TC, TG, HDL-C, LDL-C, MDA, SOD in serum as well as TC, TG in liver were assayed the 63rd day. Meanwhile, the impacts to hepatic hemorheology, pathology and artery were also examined. In the therapeutic study, affer the rats were raised with high fat diet for successive 3 weeks, they were administered with various doses of α-amylase inhibitor for the next 3 weeks. The levels of TC, TG, HDL-C, LDL-C in serum and TC, TG in liver were determined in the 42nd day. RESULTS： In the prevention study, α-amylase inhibitor of the middle and high doses could markedly decrease the concentration of TC, TG, LDL-C and MDA in serum as well as TC, TG in liver. It increased the level of HDL-C and the activity of SOD. The treatment also resulted in improvement in hepatic hemorheology, pathology and artery. In the therapeutic study, α-amylase inhibitor of the high doses could significantly reduce the concentration of TC, TG and LDL-C in serum as well as TC, TG in liver. But the treatment increased the concentration of HDL-C in serum. CONCLUSION： α-amylase inhibitor could regulate blood lipid, prevent hyperlipidemia and atherosclerosis. It also has certain effeets on formed hyperlipemia.