HPLC法测定洁阴净栓中苦参碱和连翘苷的含量

Determination of matrine and phillyrin in Jieyinjing Suppositories by HPLC

目的:建立洁阴净栓(苦参、连翘、黄柏、百部等)中苦参碱和连翘苷的HPLC方法.方法:采用HPLC法测定其含量,选用ODS色谱柱,苦参碱以甲醇-0.02%三乙胺溶液(55:45)为流动相,检测波长220 nm;连翘苷以乙腈-水(27:75)为流动相,检测波长277 nm.结果:苦参碱和连翘苷在9.8μg·mL-1～49.0μg·mL-1(r=0.999 96)和5.6μg·mL-1～56.0μg·mL-1(r=0.999 9)范围内线性关系良好,平均回收率为99.48%和100.07%,RSD分别为1.49%和1.34%(n=5).结论:本法操作简便、灵敏、准确,可用于洁阴净栓的质量控制.

AIM ： To establish the content determination method of matrine and phillyrin in Jieyinjing Suppositories （Radix Sophorae Flavescentis, Fructus Forsythiae, Cortex Phellodendri Chinensis, Radix Stemonae, etc. ） by HPLC. METHODS： HPLC was performed to determine matrine on ODS column, the mobile phase consisting of methanole -0.02% triethylamine （55： 45） and detection wavelength at 220 nm and the phillyrin of mobile phase consisting of acetonitrile-water （25： 75 ） and its detection wavelength at 277 nm. RESULTS ： The linearity of matrine was in the range of 9.8μg·mL^-1-49.0μg·mL^-1（ r=0.999 96） , and the average recovery was 99.48% , RSD = 1.49% ; The linearity of phillyrin was in the range of 5.4μg·mL^-1-54.0μg·mL^-1（r =0. 999 9） , and the average recovery was 100.07% , RSD=1.34%. CONCLUSION： This method is simple, sensitive and accurate, and available for control of Jieyinjing Suppositories.