脑室注入高浓度铁离子诱发大鼠神经细胞凋亡

Apoptosis of neurons induced by higher concentration iron in cerebrospinal fluid of rats

目的研究脑脊液中铁离子对神经元的作用,探讨铁离子与老年性痴呆症的关系。方法将FeCl3(5.2μg/μl)溶解于人工脑脊液中,取10μl缓慢注入脑室,对照组只给等量的人工脑脊液,第5天、第10天重复输注,第10天将脑组织取出,用流式细胞仪测定凋亡细胞百分比及平均荧光强度。结果脑室注入铁离子组凋亡细胞发生的百分比(14.27±0.4)%明显高于对照组(3.2±0.19)%(P<0.05)。结论脑脊液中高浓度铁离子能促进神经元凋亡,考虑到神经元凋亡是老年性痴呆(AD)的特征性病变,脑脊液中高浓度铁离子可能与散发性老年性痴呆有关。

Objective To study the relationship between concentration iron in cerebrospinal fluid and apoptosis of neurons. Methods FeCl3 （5.2 μg/μl）dissolved in simulacrum of cerebrospinal fluid was injected into rats＇ cerebral ventricle slowly , while only the same volume of simulacrum of cerebrospinal fluid to control group. The above treatment was repeated at d 5 and 10. All neurons were loaded with,annexin V-FITC, while the percentage of early apoptosis in neurons and fluorescence intensity（FI） of neurons were detected by flow cytometry. Results The percentage of apoptosis neurons in treatment group was significantly higher than that in control group. Conclusion Considering the apoptosis of neurons is a main pathologic change in AD, the higher concentration of iron in BCF may be involved in sporadic AD.