重复经颅磁刺激对脑出血家兔血肿周围组织中化合物含量的影响

Effects of repetitive transcranial magnetic stimulation on content of perihematoma chemical substance in rabbits with cerebral hemorrhage

目的观察重复经颅磁刺激(rTMS)对脑出血家兔血肿周围组织化合物含量的影响,并探讨其治疗机制。方法家兔36只,随机分为治疗组、模型组和对照组。治疗组和非治疗组采用兔自体血注射制作脑出血模型,对照组注射生理盐水。治疗组家兔于造模后12h开始实施rTMS,每日3次;模型组和对照组不进行rTMS。分别于造模后12h、3d、1周和2周,应用1H-磁共振波谱(1H-MRS)检测出血灶周围脑组织N-乙酰天门冬氨酸(NAA)、总肌酸(Cr)和胆碱复合物(Cho)等化合物的含量。结果与对照组比较,模型组和治疗组脑出血灶周组织内NAA和Cr含量减少,NAA/Cr降低,Cho含量增加,Cho/Cr增高(均P<0.01);与模型组比较,治疗组NAA、Cr含量增加,NAA/Cr增高,Cho含量减少,Cho/Cr降低(均P<0.01)。结论rTMS可通过促进血肿周围神经元的修复,影响脑组织中化合物含量,发挥神经保护性治疗作用。

Objective To investigate the effects of repetitive transcranial magnetic stimulation （rTMS） on chemical substance contents in the perihematomal brain tissues in rabbits with cerebral hemorrhage and the mechanisms underlying it. Methods Thirty-six rabbits were divided randomly into 3 groups, group A （artificial cerebral hemorrhage followed by rTMS） and group B （artificial cerebral hemorrhage without rTMS） were experimental groups and group C was the normal control group. The cerebral hemorrhage model was made by injection of self-body blood, and the control group was made by injection of saline. The rabbits of group A were treated with rTMS 3 times a day and the others received no stimulation. Rabbits in all 3 groups were tested in terms of the contents of N-acetylaspartate （NAA）, choline （Cho） and creatine （Cr） in the perihematomal brain tissues by using the technique of ^1H-magnetic resonance spectroscopy （ ^1H-MRS） at 12 h, 72 h, 1 w and 2 w after the model was established. Results Compared with group C, the contents of NAA, Cr, NAA/Cr decreased and Cho, Cho/Cr increased significantly in both groups A and B（P 〈0.01）. Compared with group B, the contents of NAA, Cr, NAA/Cr increased and Cho, Cho/Cr decreased significantly of group A （P 〈0.01）. Conclusion The results suggested that rTMS could bring about therapeutic neuroprotection effect via facilitating the neuronal recovery and influencing the chemical substance contents in perihemotomal brain tissues.