低频磁刺激对PC12细胞分化的影响

The effect of low-frequency magnetic stimulation on PC12 cell differentiation

目的探讨磁刺激对大鼠肾上腺嗜铬细胞瘤细胞株(PC12)分化及功能的影响。方法应用Magstim220型磁刺激仪分别刺激单纯PC12细胞组(M组)和PC12+神经生长因子(NGF)组(MN组),刺激强度为0.38T、1.14T、1.9T(总强度1.9T),频率为1Hz,每天于10s内连续刺激10次,连续刺激9d。每隔1d观察细胞增殖和突起生长的情况,于倒置显微镜下随机取20个视野拍照并计数,于第3天、第6天、第9天留取细胞培养液检测多巴胺水平。结果在1.14T刺激强度下细胞突起显著增加;多巴胺水平在刺激强度为0.38T时达高峰。结论低频磁刺激可促进PC12细胞的分化及多巴胺分泌;1.14T、1.9T刺激强度时联合应用NGF可提高PC12细胞的分化。

Objective To study the effect of low-frequency magnetic stimulation （MS） on differentiation on PC12 ceils. Methods The PC12 cells were divided into two groups, one was treated with magnetic stimulation only, the other were treated with magnetic stimulation plus administration of nerve growthfactor（NGF）. The cells were subject to 10 seconds of magnetic stimulations once daily at a frequency of 1 Hz and intensities of 0.38 T, 1.14 T and 1.9 T, respectively, for 9 days. The proliferation and neurite extension of PC12 cells was observed by inverse microscopy every other day. Dopamine （DA）level in the culture medium was detected on the 3rd,6th and 9th days. Resuits There was significant increase in enation of PC12 cells in the 1. 14 T stimulation group. MS treatment increased the extracellular DA levels, which was peaked in the 0.38 T stimulation group. Conclusion Low-frequency magnetic stimulation of the PC12 cells might facilitate cell differentiation and induce the release of endogenous dopamine. Magnetic stimulation of 1.14 T in combination of NGF could improve PC12 cell differentiation.