p53、bcl-2和CD44v6蛋白表达与乳腺癌转移的相关性研究

The Study of Correlation between p53 bcl-2 CD44v6 Expression and Metastasis of Breast Cancer

目的探讨乳腺癌及癌旁正常乳腺组织中p53、bcl2和CD44v6蛋白的表达及意义。方法采用免疫组化法检测96例乳腺癌及其癌旁正常乳腺组织中p53、bcl2和CD44v6蛋白的表达,并分析其与淋巴结转移和cerbB2表达状态的相关性,从而评价这些指标在预测乳腺癌转移方面的价值。结果正常乳腺组织中p53蛋白为阴性,在乳腺癌中阳性表达率为65.63%;随着组织学分级的增高,阳性率逐渐增高;淋巴结转移组阳性表达率明显高于无淋巴结转移组,并与cerbB2表达状态呈正相关。乳腺癌组织中bcl2阳性表达率明显低于周围正常乳腺组织,随着组织学分级的增高,阳性率逐渐降低,淋巴结转移组中的表达率明显低于淋巴结非转移组,与cerbB2的表达呈负相关。CD44v6在乳腺癌组织中的阳性表达率明显高于正常乳腺组织,随着组织学分期的增加,CD44v6的阳性表达率亦增高,但差异无显著性,淋巴结转移组的阳性率略高于无淋巴结转移组,差异也无显著性,与cerbB2表达无相关性。结论p53和bcl2蛋白可作为预测乳腺癌转移的指标,CD44v6的阳性表达可能与乳腺癌的发生、进展有一定关系,但尚不能把它作为预测乳腺癌转移的稳定的生物学指标。

Objective To investigate the expression and significance of p53, bcl-2 and CD44v6 in breast cancer and normal breast tissue beside cancer tissue. Methods Expression of p53, bcl-2 and CD44v6 were tested in 96 cases by immunohistochemical methods. The significance of these markers was evaluated by analyzing the correlation between these markers and lymph node metastasis, expression of c-erbB-2. Results p53 was negative in normal breast tissue while was positive in 65.63% cancer tissue. There was a significant positive correlation between expression of p53 and histological grade, lymph node metastasis, expression of c-erbB-2. Expression of bcl-2 in breast tissue was significantly lower than that in normal tissue and a negative correlation was found between expression of bcl-2 and histological grade, lymph-node metastasis, expression of c-erbB-2. Expression of CD44v6 in breast tissue was significantly higher than that in normal tissue and no correlation was found between expression of CD44v6 and histological grade, lymph-node metastasis, expression of c-erbB-2. Conclusion p53 and bcl-2 can be used as important metastasis markers for breast cancer, while CD44v6 can not be regarded as a stable biological symbol of metastasis even though it also has some relationship with breast cancer yet.