反复热性惊厥过程中γ-氨基丁酸B受体对气体信号分子一氧化碳的影响

Influence of Gamma-Aminobutyric Acid B Receptor on Expression of Carbon Monoxide/ Heme Oxygenase System during Recurrent Febrile Seizures

目的探讨γ氨基丁酸B受体(GABABR)对热性惊厥(FS)大鼠一氧化碳(CO)/血红素氧合酶(HO)系统表达的影响。方法SD大鼠32只随机分为对照组、FS组、FS+baclofen组、FS+phaclofen组,每组各8只。采用热水浴诱导大鼠FS,隔日诱导1次,共10次。采用双波长分光光度计法测定大鼠血浆CO含量;用原位杂交观察GABABR和HO1mRNA表达情况;用免疫组织化学方法观察GABABR和HO1蛋白表达情况。结果FS+baclofen组CO含量高于FS组,同时HO1表达也较FS组增强;而FS+phaclofen组CO含量低于FS组,同时HO1表达也较FS组减弱。FS+baclofen组和FS+phaclofen组与FS组相比,差异均有显著意义(P均<0.05)。结论反复FS过程中,GABABR的改变可影响CO/HO1系统的表达。

Abstract： Objective To explore the influence of γ-aminobutyric acid B receptor（GABABR）on carbon monoxide （CO）/heme oxygenase（HO - 1 ）system during recurrent febrile seizures （FS）. Methods Sprague Dawley rats aged 21 days were randomly divided into 4 groups： control group and FS group, FS ＋ baclofen group, FS ＋ phaclofen group. FS in rats were induced 10 times in a bath of wama water, once every 2 days. The plasma level of CO was detected by the dual wave lengh spectrophotometer; the expressions of GABABR and HO 1 mRNA were examined by insitu hybridization; the expressions of GABABR and HO- 1 protein were observed by immunohistochernistry. Results The plasma level of CO increased in FS ＋ baclofen group, while decreased in FS ＋ phaclofen group compared with FS group, The expressions of GABABR and HO 1 upregulated in FS＋ baclofen group, while decreased in FS ＋ pha- clofen group compared with FS group. There were significant difference （All P〈 0.05）. Conclusion GABABR can modulate the expression of CO/HO- 1 system during recurrent FS.