冠心病患者凝血因子Ⅶ-3230/10bp基因多态性的检测

Detection of-323 0/10 bp polymorphism of factor Ⅶ in the patients with coronary heart disease

目的:研究冠心病(CHD)患者凝血因子Ⅶ(FⅦ)-323 0/10 bp基因多态性的频率及意义。方法:活化FⅦ(FⅦa)测定采用重组可溶性组织因子一期法,FⅦ活性(FⅦc)测定采用凝血一期法,FⅦ总抗原(FⅦag)采用酶联免疫吸附法(ELISA法)。多态性分析用PCR-尿素聚丙烯酰胺凝胶电泳法。结果:60例FⅦ-323 0/10 bp多态性检测发现5例0/10 bp多态性,未检出10/10 bp多态性。10 bp等位基因检出率为8%。在存在0/10 bp基因多态性的病例组的FⅦc,FⅦag比0/0 bp组明显减低。结论:FⅦ-323 0/10 bp多态性存在于中国的CHD患者中,它与CHD血浆FⅦc、FⅦag水平有关;患者10 bp等位基因是CHD血栓形成的保护因素。

Objective：To study the gene frequency and meaning of -323 0/10 bp polymorphism of factor NⅡ （FNⅡ ） in coronary beart disease （CHD）. Method：FNⅡa was determined with one stage clotting assay using a recombi nant soluble tissue factor（rsTF） . FNⅡc was measured with one stage clotting assay. FNⅡag was quantified with an enzyme-linked immunosorbent assay （ELISA）. Gene polymorphism was identified by carbamide-polyacrylamide gel electrophoresis. Result：Five 0/10bp polymorphism was identified by the analysis of FNⅡ-323 0/10 bp polymorphism. In the patients with 0/10 bp polymorphism, FNⅡc and FNⅡ ag were obviously lower than those with 0/ 0 bp polymorphism. Conclusion：- 323 0/10 bp polymorphism of FVNⅡ can be detected in Chinese CHD patients, which is possibly related to the increase of FNⅡc and FNⅡag in CHD. The 10 bp allele gene is a protective factor of thrombogenesis in CHD.