人参皂苷Rb1对缺氧复氧诱导内皮细胞凋亡的影响

Effect of ginsenoside on endothelial cells apoptosis induced by hypoxia-reoxygenation

目的:观察缺氧复氧诱导人脐静脉内皮细胞凋亡现象及人参皂苷Rb1的干预效应。方法:体外培养人脐静脉内皮细胞,建立内皮细胞缺氧复氧模型,采用TUNEL技术和DNA凝胶电泳观察缺氧不同时间(0、3、6、12、24 h)后复氧对内皮细胞凋亡的影响及Rb1的干预效应。结果:随着缺氧时间延长,复氧后脐静脉内皮细胞凋亡率逐渐升高;Rb1组内皮细胞凋亡率显著低于缺氧复氧组(P<0.05)。结论:内皮细胞凋亡是缺氧复氧损伤的一种重要形式,人参皂苷Rb1通过抑制缺氧复氧诱导内皮细胞凋亡而起到内皮细胞保护效应。

Objective：To observe the endothelial cells apoptosis induced by hypoxia and reoxygenation and the inhibiting effect of ginsenoside Rbl. Method： Human umbilical vein endothelial cells （HUVECs） were cultivated and divided into five groups randomly, which were used to establish the hypoxia-reoxygenation models. TUNEI. technology was used to quantify the apoptotic and necrotic cells. DNA electrophoresis was utilized to determine changes characteristic of apoptosis. Result： Percentage of apoptosis in different groups was significantly distinctive, which increased steadily with the duration of hypoxia reoxygenation, apoptic cells in Rbl group were significantly less than in the hypoxia-reoxygenation group （ P〈 0.05）. Conclusion： Hypoxia-reoxygenation could initiate apoptosis in cultivated HUVECs in a time dependent manner. Rbl exerts cell protection in hypoxia reoxygenation injury by inhibiting endothelial cells apoptosis.