低密度脂蛋白免疫复合物对单核细胞来源的巨噬细胞脂质代谢的影响

Effects of LDL-containing immune complexes on lipid metabolism in monocyte-derived macrophages

目的:研究动脉粥样硬化过程中低密度脂蛋白免疫复合物(LDL-IC)对单核细胞来源的巨噬细胞内脂质含量的影响。方法:以佛波酯刺激分化的THP-1细胞作为单核细胞来源巨噬细胞模型。固定后的细胞以苏丹Ⅳ染色,观察细胞内脂质含量变化。通过胆固醇酶联法测定细胞内胆固醇含量。结果:与LDL-IC孵育能使单核细胞来源巨噬细胞内胆固醇堆积,呈现泡沫细胞的外观。细胞内胆固醇水平随着LDL-IC的浓度增加与作用时间的延长而升高。在4组平行对照实验中,LDL-IC组细胞内胆固醇水平明显高于阴性对照组、LDL组和IgG-IC组(P<0.01)。结论:LDL-IC能通过增加细胞内脂质而在动脉粥样硬化过程中发挥作用。

Objectlve：To study the effects of LDL containing immune complexes （LDL-IC） on the lipid metabolism in monocyte derived macrophages. Method：We used PMA-treated THP 1 cells as a model of monocyte derived macrophages. The fixed macrophages were stained by sudan IN and quantitative analysis of intracellular cholesterol content was performed by cholesterol enzyme assays. Result：Incubating with LDL-IC led to intracellular cholesterol accumulation and formation of foam cells. The intracellular cholesterol levels were correlated with the concentration of LDL- IC and incubating time. The intracellular cholesterol level of the macrophages treated with LDL-IC was higher than that in other control groups （ P〈0.01 ）. Conclusion： LDL-IC may play a role during the development of atherosclerosis through the way of increasing intracellular lipid level.