新疆南部地区蜱传虫媒病毒分子生物学调查

Molecular biological survey of tick-born arboviruses in southern part of Xinjiang

目的了解新疆南部地区蜱传虫媒病毒的种类和分布情况。方法在新疆南部地区的23个采集地36个采集点,采集蜱标本5045只,分别建立蜱标本cDNA文库,用PCR方法检测标本可能携带的病毒。结果黄病毒属引物、加利福尼亚血清组病毒引物对34份cDNA文库检测结果均为阴性;内罗毕病毒属引物和新疆出血热病毒巢式引物在巴楚县蜱标本中检测到新疆出血热(XHF)病毒核酸序列,对检测到的XHF病毒L和S片段进行系统进化分析。结论对新疆南部地区5045只蜱进行四种六对引物的PCR检测,未检出黄病毒属和加利福尼亚血清组病毒核酸序列,获得新疆出血热病毒L和S片段的核酸序列,研究了其分子流行特征。

Objective To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang. Method Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang, which were made into cDNA pools with pd （N）6 primer through RT-PCR method. Then PCR was used to detect viral nucleotide sequence from cDNA. Result All 34 cDNAs showed negative to flavivirus and California serogroup virus primers; but nairovirus and primets derived from Xinjiang hemorrhagic fever virus had amplified and yielded some obvious bands corresponding to the nucleotide sequences of Xinjiang hemorrhagic fever virus. A phylogenetic analysis was done to the obtained partial sequences of L and S segments. Conclusion Nucleotide sequences of Neither flaviviruses nor California serogToup viruses were detected from the samples. However partial L segment sequence was first reported in China. Phylogenetic analysis of partial L and S segments disclosed the molecular characteristic of Xinjiang hemorrhagic fever virus.