耐高温耐高酒度醋酸菌选育研究

Studies on the Breeding of Thermotolerant and High Ethanol Tolerant Acetic Acid Bacteria Strain

从高温季节发酵良好的醋醅中,分离筛选出N 9、F17两株醋酸菌,在培养温度35℃,培养基酒度6%(V/V)条件下,产酸量均高于A S1.41号.以N 9、F17为出发菌株,经紫外线、硫酸二乙酯逐级诱变,分别获得编号为HN 15、HF6两株耐高温耐高酒度醋酸菌株.在培养温度40℃,培养基酒度12%(V/V)条件下,HN 15酒精转化率为75%,HF6酒精转化率为70%.

Two acetic acid bacteria N9 and F17 were obtained by isolation and screening in the medium of vinegar solid state fermentations. The productivities of acetic acid of N9 and F17 are higher at 6%（V/V） ethanol content and 35℃ than the strain of AS1.41. Mutant strain HN15 was obtained from strain N9 and mutant strain HF6 was obtained from strain F17 by UV and DES treatment. The ethanol oxidation activity of strain HN15 is 75% and that of strain HF6 is 70% at 12%（V/V）ethanol content and 40℃.