补冲丸对肾阳虚型黄体功能不全患者子宫内膜雌孕激素受体影响

Influence on Endometrium Female Hormone and Progesterone Receptor of Luteal Phase Defect with Deficiency of Kidney-Yang Patients with Buchong Pills

[目的]探讨中药治疗肾阳虚型黄体功能不全的作用机制。[方法]采用SP免疫组化检测技术、电化学发光法对30例患者治疗前后子宫内膜雌孕激素受体、血清性激素进行定量检测。[结果]治疗前30例中有16例患者血清孕酮在正常值(10ng/mL)水平以上,但子宫内膜分泌不良,同时伴有雌二醇(E2)、孕激素受体(PR)下降,治疗后E2水平上升,PR增加,经统计学处理差异显著(P<0.05),并且有6例妊娠。另14例患者孕激素(P)水平低于正常值,子宫内膜雌激素受体(ER)、PR与对照组无显著差异,但E2水平低于对照组(P<0.05),治疗后,E2水平及孕酮P水平显著上升(P<0.05),5例妊娠。[结论]补冲丸具有弱雌激素样作用,通过调节下丘脑—垂体功能,进而改善卵巢分泌E2、P的作用,并能提高患者子宫内膜PR含量,从而改善子宫内膜分泌状况,使患者妊娠。

[Objective] To explore the effect mechanism of luteal phase defect with deficiency of kidney-yang syndrome on traditional Chinese herbs. [Methods] The seventy patients were divided randomly into treatment group and control group. The patients of treatment group were administrated by Buchong pills and that of control group were administrated by allylestrenol. To determine the endometrium female and progesterone receptor and serum gonadal hormone content before and after the treatment by using SP immunohistochemical determination technique and electrochemiluminescence method. [Results ] The sixteen patients of treatment group was higher than the normal level on serum progesterone level and fourteen patients of treatment group was lower than the normal level on serum progesterone level. The E2 and PR level of treatment group before the treatment was obviously different than that of treatment after the treatment （P〈0.05）. And there were eleven fetation patients in the course of treatment. [Conclusion ] The Buchong pills may enhance PR content of endometrium, improve the externalization condition of endometrium and cause to pregnancy by regulating hypothalamus and appendix cerebri function. The Buchong pills had similarly inferior female-hormonelike effect.