HPLC法测定小蓟中绿原酸的含量

Determination of Content of Chlorogenic Acid in cephalanopli herba by HPLC Method

[目的]研究小蓟中绿原酸简便快速的含量测定方法。[方法]采用高效液相色谱法,测定2种产地小蓟中绿原酸的含量。色谱条件:LichrospherC18色谱柱(4.6mm×200mm,5μm),柱温30℃,以甲醇-1%醋酸(15∶85)为流动相,流速1.0mL/min,检测波长327nm。[结果]绿原酸在0.00328～0.02952μg/μL范围内具有良好的线性关系(r=0.9997),平均回收率为100.12%,RSD=2.36%。[结论]经过系统的方法学考察,该方法具有简便、稳定、可重复的特点,可用于小蓟中绿原酸的含量测定。

[Object] To study conveniently and rapidly determination method of chlorogenic acid content in cephalanopli herba. [Method] To determine the chlorogenic acid content in three different metropolis of cephalanopli herba by using HPLC method. The chromatographic conditions were： Lichrospber C18 column（4.6mm×200mm,5μm）, column temperature at 30℃, methanolacetic acid （15：85）as mobile phase of chlorogenic acid, flow rate 1.0 mL/min, detection wavelength at 327 rim.[Results] The liner range of chlorogenic acid was 0.003 28-0.029 52 μg/μL （r=0.999 7）, the average recovery was 100.12% and RSD was 2.36. [Conclusion] The method was proved to be simple,rapid, and accurate and may be used to determine chlorogenic acid content in cephalanopli herba.