紫草细胞培养液中色素提取方法的研究

Study on The Methods of Recovering Shikonin Derivatives From Lithospermum Erythrorhizon Cell Cultures Medium

紫草细胞培养生产紫草色素，有一部分紫草色素外泌到培养液中．由于培养液体积大，产物含量低，因此如何将色素加以回收是该项技术产业化的关键之一．本文分别采用萃取法、沉淀法和吸附法研究色素回收问题．发现用沉淀法和吸附法代替传统萃取法提取培养液中的紫草色素，可使紫草细胞培养生产紫草色素产物提取等下游过程工艺简化，收率提高，成本降低．

Cell suspension cultures of lithospermum erythrorhizon produce a large quantity of shikonin derivatives (red naphthoquinone pigments) and a part of them are se creted into the medium. Because of the large volume of liquid medium and the lowconcentration of product in it, pigments recovery from medium becomes one of thekey problems in industrializing this technology. In this paper,three isolation methods──extraction, precipitation and adsorption were used to recover pigments from medium.It resulted that in comparision with extraction, the tradrtional method, precipitation and adsorption simplified the downstream proccess,increased the recovery and accordingly the production cost was cut down greatly.