绵羊夏伯特线虫外生性发育的研究

Cultivation and Devdopment of Sheep Chabertia ovina egg in vitro

把绵羊夏伯特线虫（Ｃｈａｂｅｒｔｉａｏｖｉｎａ）虫卵置于消毒粪便培养基内，于２５℃温箱内及１７℃（±２℃）室内进行培养。结果表明：在２５℃下经４０～４４ｈ第一期幼虫逸出卵壳，６２～６９ｈ完成第一次蜕皮成为第二期幼虫，１６２～１８９ｈ完成第二次蜕皮成为第三期幼虫；第三期幼虫肠细胞呈矩形，共３２个，尾鞘长０．１７５～０，２２０ｍｍ，生殖原基距头端０．３４５～０．３８１ｍｍ；在室内培养的虫卵发育缓慢，约经１０ｄ才发育为第三期幼虫。

The eggs of Chabertia coina in sheep faceal cultures were cultured at 25℃ hothouseand at room temperture(17±2℃).Eggs hatch into lst stage larvae in 40 44 hours at 25℃ andmolt to the 2nd stage larvae in 62 69 hours and molt to 3rd stage in 162 189 hours.The 3rdstage larvae measures 0. 680 mm in length including the heath.The intestine is bordered by 32rectangular cells.The genital primordium is 0.345 0.38 1mm from the anterior end.The tailsheath is 0.175 0.220mm length.At a temperature of 17±2℃,eggs development was slowerand reached the 3rd stage larvae in 10 days.