大鼠肢体IRI后脊髓神经元Fos的表达

Expression of fos in Spinal Neuron after Rat Limb IRI

目的探讨脊髓神经元是否参与肢体缺血再灌注损伤(IRI)的发病过程以及有关神经元的分布特点。方法Wistar大鼠24只,随机分为4组,A:空白对照组;B:假手术对照组;C:单纯缺血组;D:缺血再灌注组。通过暂时阻断一侧髂总动脉和股动脉,建立肢体缺血再灌注损伤模型。采用免疫细胞化学ABC法,观察大鼠脊髓神经元原癌基因c-fos的表达和分布特点。结果大鼠肢体缺血4h,脊髓相应节段神经元Fos表达活跃。再灌注2h后,Fos表达更加活跃(P<0.01)。并且Fos阳性神经元较集中分布于脊髓相应节段同侧后角及中央管周围。结论脊髓相应节段同侧神经元可能参与肢体IRI的发病过程,尤其是脊髓后角及中央管周围神经元可能具有更为重要的作用。

Objective Limb ischemia-reperfusion injury, which can be caused by many kinds of injuries and diseases, is a clinically common pathological process , usually accompanied with a high rate of disablement. Present researches concerning about it focus more on skeletal muscles than on nerve system. Methods 30 Wistar rats are divided into 4 groups at random： group A is the controls; group B were operated only; group C of 4 hours ischemia only and group D of 4 hours ischemia followed by 2 hours reperfusion; The model is build through the temporary occlusion of the left common iliac and femoral arteries. Immunocytochemical methods are used to observe the expressions of c-los and their distributions in spinal cord of the rats. Results We found that the expressions of c-los are enhanced in the spinal cord of the rats followed by 4 hours limb ischemia. After 2 hours reperfusion, the above enhancement becomes more evident （ P 〈0. 01）. And the majority of Fos positive neurons locates in the posterior horn and around the central canal. Conclusions The results indicate that the neurons in correlating segments of spinal cord may be participating in the limb ischemia-reperfusion process , especially those locating at posterior horn and around central canal.