钙通道阻滞剂Nifedipine对自发性高血压大鼠血管内皮细胞的保护作用

Protective effect of calcium channel blockers Nifedipine on vascular endothelial cells for spontaneous hypertension in rats

目的观察钙通道阻滞剂Nifedipine控释剂对自发性高血压大鼠(SHR)一氧化氮(NO)及诱导性一氧化氮合酶(iNOS)的影响。方法本实验为随机对照的实验研究,在山东大学医学院实验室完成。实验动物为近交SHR大鼠21只。实验动物被随机分3组:生理盐水对照组7只,Nifedipine正常剂量组7只,Nifedipine低剂量组7只。每天灌胃一次,连续15 d,末次给药后摘眼球取血并取大鼠心、肺分别测定血清NO和iNOS的含量。结果:灌胃15 d后,正常剂量组的NO含量为(135±7.7)ptnol/L,与生理盐水组(102.3±4.6)ptnol/L相比,差异有显著性意义(P<0.01);正常剂量组心、肺组织块中iNOS活性降低,为(1.15±0.18),差异有显著性意义(P<0.01);与低剂量组(1.77±0.23)相比,差异亦有显著性意义(P<0.05)。结论Nifedipine能在有效降低血压的同时,提高血清NO的含量,并且对抗血压增高所造成的iNOS的活性增强(或二者互为因果)。

Objective： To study the effect of Nifedipine on nitric oxide（NO） and inducible nitric oxide synthase（iNOS） in spontaneous hypertension rats. Methods： A randomly controlled trial study was made. The research was accomplished in Shandong University Medical College. Twenty-one spontaneous hypertension rats were divided into the control group, normal and low doses Nifedipine groups. The drugs were administered once a day by gastrogavage for 15 successive days. Blood was collected from the eye-balls and the viscera pulmo to determine the NO and iNOS in the serum after the last time. Results： The NO in the serum in the normal dose group was 135±7.7μmol/L, and 121±6.4μmol/L in the low dose group, which were both significantly higher than those in the control group （P 〈 0.01 and P 〈 0.01）. It was also observed that the iNOS activity was significantly depressed in the normal dose （1.15±0.18） and the low dose （1.77±0.23） Nifedipine groups than that in the controls （2.43±0.76） （P 〈 0.05）. Conclusion： Nifedipine can low the pressure and at the came time can raise NO in the serum and depress the activity of iNOS caused by the hypertension.