水稻高压诱变与突变体的ISSR分析

Pressure Induced Rice Mutation and ISSR Analysis of the Mutants

以两个水稻(Oryza sativaL.)品种粤香占和毕粳38号为材料,通过高压处理,获得了5个当代突变体和4个后代分离出的突变体。ISSR分析表明高压可以诱导水稻产生变异,这种变异是由于遗传物质(DNA)的改变所引起的。由高压诱变产生的水稻突变体不仅在当代出现,而且具有很高的遗传稳定性,突变体与原种的遗传差异越大,其稳定性越高。

After up to 10 generations of purification by strict self-fertilizing, seeds of two rice varieties （Oryza sativa L.）, Yuexiangzhan and Bijing-38 were treated with high hydrostatic pressure during last 5 years, and 5 contemporaneous mutants and 4 descendant natural mutants were obtained with high reproducibility for the contemporaneous mutants. To verify if the mutation was take place at DNA level, Inter Simple Sequence Repeat （ISSR） analysis of the original rice varieties and mutants was conducted. The analysis revealed that the genetic substance （DNA） had been altered. Evidently, the mutation was induced by high hydrostatic pressure treatment. Generally, the mutation took place contemporaneously and the new characters are of high genetic stability; the further the genetic distance between the mutant and the original is, the higher the genetic stability of the mutant is.