稳定同位素标记方法在蛋白质组学定量分析中的应用

Application of Quantitative Proteomics Expression Analysis Using Stable Isotope Labeling

蛋白质表达水平定量分析是蛋白质组学研究的一项关键内容,它依赖于精确的、高通量的、具重复性的技术。目前,应用稳定同位素标记技术定量蛋白质表达水平是最准确的方法之一,且联合色谱质谱技术更具优势。本文论述了这一技术的广泛应用和最新的研究进展。

Quantitative protein expression profiling is a crucial part of proteomics and requires technique that are able to efficiently provide accurate, high-throughput and reproducible differential expression values for proteins in two Or more biological samples. At present , stable isotope labeling is probably considered as one of the most accurate ways to relatively quantify protein expression levels and additionally stable isotope labeling may be directly combined to LC MS/MS approaches. In summary,this technique has its advantages in quantitative proteomics. The application and the latest progresses about this technique are discussed.