葡萄籽提取物对人二倍体成纤维细胞寿命及c-fos基因表达的影响

EFFECTS OF GRAPE SEED EXTRACT ON LIFE-SPAN OF HUMAN DIPLIOD FIBROBLAST AND TRANSCRIPTION OF C-FOS GENE

研究葡萄籽提取物对人二倍体成纤维细胞寿命及原癌基因c-fos转录的影响,探讨葡萄籽抗衰老的细胞和分子基础.采用体外培养细胞、Northern杂交RNA电泳及细胞染色体常规检查的方法进行检测.葡萄籽提取物在体积分数为0.030%时,能有限地延长2BS细胞的寿命;葡萄籽提取物能诱导衰老2BS细胞c-fos基因的低水平转录.葡萄籽具有一定的延缓细胞衰老的作用,其对衰老2BS细胞c-fos基因转录的诱导作用,可能是延缓细胞衰老的分子机理之一.

The effects of grape seed ertract on the life - span of human diploid fibroblast and transcription of c - fos on cogene are explored , and the molecular and cellular mechanism of anti - aging of grape seed extract is also investigated by cell cultare, Northern blotting, RNA electrophoresis and regular analysis way of cellular chromatosome. The experimental results show that grape seed extract can exterd the life - span of diploid fibroblast and induce the transcription of c - fos on cogene of aging diploid fibroblast when the concentration of grape seed extract is 0.030%. It is conduded that grape seed extract has ability to slow down the cell aging, and the inducton of grape seed extract on the transcription of aging 2BS cell＇s c -fos gene may be one of molecular mechanism of anti -aging cell.