肝硬化患者血清中一氧化氮和IL-10水平与病情变化关系探讨

Changes of NO and IL-10 in sera from patients with hepatic cirrhosis

目的探讨血清中一氧化氮(NO)和白介素10(IL10)水平的变化与肝硬化病情演变过程的相互关系及其可能的作用机制。方法应用硝酸还原酶法和ELISA法测定36例活动期和恢复期肝硬化患者,38例活动期和恢复期慢性肝炎患者,38例活动期和恢复期急性肝炎患者及30例正常人血清中NO和IL10水平的变化。结果肝硬化患者血清中NO水平与其他三组比较显著升高,恢复期患者也不能降至正常水平(P<0.01);而IL10水平明显低于对照组(P<0.01)。活动期的慢性肝炎患者和急性肝炎患者血清中NO水平与正常组比较有不同程度的升高(P<0.01),但不如肝硬化组患者显著(P<0.01)。急性肝炎患者恢复期血清中NO水平可降至正常水平。慢性肝炎患者和急性肝炎患者活动期血清中IL10水平也表现为不同程度的降低。相关性分析结果显示:肝硬化患者血清中NO水平与IL10水平呈明显负相关(r=-0.469,P<0.01)。结论IL10和NO参与肝硬化病情演变机制,并可作为检测病情演变的可靠指标之一。

Objective To investigate the changes and their possible mechanism of NO and IL-10 in sera from patients with hepatic cirrhosis and to analyze their correlation with the disease activity. Methods Sera from 39 patients with active and convalescent hepatic cirrhosis, 38 patients with active and convalescent chronic hepatitis, 38 patients with active and convalescent acute hepatitis and 30 normal controls were tested for NO and IL-10 with ELISA assay. Results Compared to the other three groups, level of NO in patients with hepatic cirrhosis was significantly elevated, and was not able to decrease to normal level even in convalescent phase （P〈0. 01）; IL-10 level in patients with hepatic cirrhosis decreased significantly （P〈0.01）. Levels of NO in patients with active and convalescent acute or chronic hepatitis were also elevated, but were not as high as that in patients with hepatic cirrhosis （P,0.01）. NO in patients with acute hepatitis in its convalescent phase may be at normal level. IL-10 level in patients with active chronic and acute hepatitis decreased. Correlation analysis data showed that levels of NO correlated negatively with IL-10 in patients with hepatic cirrhosis （r=-0. 496, P〈0.01）. Conclusion NO and IL-10 were involved in the formation and developing of hepatic cirrhosis, and the detection of NO and IL-10 may be one of the reliable methods in evaluation of desease activity.