釉基质蛋白对牙周膜成纤维细胞增殖活性的影响

The effects of enamel matrix proteins on cell proliferation of cultured human periodontal ligament cells

目的:通过检测釉基质蛋白(Enamel matrix proteins,EMPs)作用下对促进牙周膜成纤维细胞增殖活性的影响,探讨EMPs促进牙周组织再生的作用机制.方法:酶消化法获得人牙周膜成纤维细胞(Human periodontal ligament cells,HP-DLC)用不同浓度的EMPs进行诱导,在3 d、7 d两个时间点,利用MTT法检测和分析诱导后细胞增殖活性的变化.结果:EMPs浓度在(50～200)mg/L时促增殖作用呈剂量依赖性.EMPs的最小显效浓度是50 mg/L,而200 mg/L EMPs的促增殖作用最强.25 mg/L与(50～200)mg/L差异显著(P＜0.05);(100～200)mg/L间无明显差异(P＞0.05).结论:EMPs在一定浓度下可以促进人PDLCs增殖.100 mg/L浓度最佳.

Objective To investigate the effects of enamel matrix proteins （EMPs） on cell proliferation by cultured human periodontal ligament cells. Methods Human periodontal ligaent cells （HPDLCs） were dissected and cultured by digestion with bacterial coUagenase. Then HPDLCs were treated with different concentration of EMPs. MTT method was used to determine the change of proliferative activity. Results EMPs（50 - 200mg/L） promoted the ability of cell proliferative activity and it depended on the denseness. The difference between the proliferative activity in 25mg/L group and 50 - 200 mg/L groups was significant, while difference between the protife rative activity in 100 and 200 mg/L was not significant. Conclusions EMPS could increase the proliferative activity of HPDLCs. 100 mg/L was the best concentration.