缺血性脑血管疾病患者血浆氨基酸递质的测定及其意义

Determination and clinical significance of amino acid transmitters in plasma of the patients with ischemic vascular disorders

目的观察血浆氨基酸递质的测定对于推测老年人脑缺血的严重程度具有临床意义。方法应用高效液相色谱法(HPLC)测定了57例老年人缺血性脑血管病患者和33名正常老年人对照血浆中兴奋性和抑制性氨基酸递质。结果在脑缺血急性期,血浆谷氨酸、天冬氨酸水平明显升高,与对照组相比分别升高了26.48%和39.70%,缺血发生24h后开始上升,恢复期降至正常水平。血浆抑制性氨基酸γ氨基丁酸、甘氨酸、丙氨酸在脑缺血急性期也升高,与对照组相比分别升高了74.57%、36.79%和21.33%。γ氨基丁酸在恢复期下降,而甘氨酸、丙氨酸未见下降。结论急性缺血性脑血管病患者血浆氨基酸递质可早期反映老年人脑缺血发生,推测老年人脑缺血的严重程度。

Objective ＇lb observe the determination and clinical significance oi amino acid transmitters in plasmas of the patients with ischemie vascular disorders. Methods Excitatory and inhibitory amino acids in plasmas of 57 old peoples with ischemic vascular disorders and 33 normal old peoples were determined with high performance liquid chromatography（HPLC）. Results Significant increases were found in plasma concentration of glutamate （ 26.48% ） , aspartate （ 39. 70% ）, γ-aminobutyric acid （ GABA, 74. 57% ）, glycine （ 36. 79% ） and alanine （ 21.33% ） within fist three days after cerebral ischemia compared to normal controls. The content of glutamate and aspartate began to increase significantly in 24 hours after ischemia. The plasma levels of glutamate, aspartate and GABA have returned to normal in recovery patients. Conclusions These results showed that the concentration changes of excitatory and inhibitory amino acids in plasmas may suggest the early happening and severity of cerebral ischemia.