乙型肝炎病毒X蛋白反式激活基因XTP13的克隆

Cloning of human gene XTP13 transactivated by X protein of hepatitis B virus

目的克隆乙型肝炎病毒(HBV)X 蛋白反式激活新型靶基因。方法以 HBV X 蛋白表达质粒 pcDNA3 1(-)-X转染 HepG2细胞,以空载体 pcDNA3.1(-)为平行对照,提取总 RNA 进行逆转录,对产物行基因表达谱芯片分析,确定新基因编码序列后,以逆转录多聚酶链反应(RT-PCR)技术扩增新基因序列。结果获得新基因的全长序列,并测序证实,命名为 X 蛋白反式激活蛋白13(XTP13)。结论成功克隆 XTP13,为进一步阐明 HBxAg 在 HBV 感染中的分子生物学机制奠定基础。

Objective To screen and clone the target gene transactivated by HBV X protein, Methods The HepG2 cells were transfected by pcDNA3.1 （-） and pcDNA3.1（-）-X,respectively. The total mRNA was isolated and reversely transcribed. The cDNA was analyzed by DNA microarray and then target gene transactivated by HBV X protein was cloned by PCR, Result One of the obtained sequences was a new gene with unknown function. It was named as XTP13, Conclusions The target gene is successfully cloned and it will pave the way for further study on the molecular mechanism of the transactivating effects of HBV X protein and the new therapy for chronic hepatitis B.