2400例宫颈细胞脱落标本HPV筛查及荧光PCR基因分型和定量

HPV screening and genotype by FQ-PCR from cervical cell in 2400 cases

目的建立快速、灵敏的HPV筛查方法及探讨HPV基因型与宫颈癌之间的关系.方法用细胞涂片法和传统PCR对2400例年龄在18～65岁的厦门郊区妇女进行筛查,对PCR阳性标本进一步进行荧光PCR基因分型和定量.结果 2400例宫颈脱落细胞普查中有宫颈原位癌4例、CIN(癌前病变)Ⅰ～Ⅲ级7例;PCR定性筛查这些标本中有84例阳性,阳性率为3.5%.对这84例阳性标本进行荧光基因分型和定量,发现13例HPV-16、18型阳性,其中HPV-16型阳性8例(占9.52%),HPV-18型阳性5例(5.95%),并且HPV-DNA拷贝数在3.6×103到5.6×107之间.在HPV-16、18型阳性的患者中宫颈原位癌3例(占75%)、CINⅠ-Ⅲ级5例(占71%).结论 HPV-16、18型与宫颈癌之间有高度相关性.

Objective：To establish a fast and sensitive method for general investigation of human papilloma virus （HPV） and study the nrelationship between the HPV genotype and the cervical cancer. Methods ： Used cytotaxonomy and traditional PCR detected 2400 women in Xiamen location .The positive samples of traditional PCR were performed the gene type and gene quantity by fluorescence quantitative PCR （FQ - PCR）. Results：Among 2400 samples,there are 4 cervical cancer cases and 7 cervical intraepithelial neoplasia （CIN） Ⅰ - Ⅲ cases analysis by cytotaxonomy. Corresponding, there are 84 cases positive by traditional PCR, the positive ratio is 3.5 %. 13 cases were HPV 16 （8 cases, 9.52% ） or 18 （5 cases, 5.95 % ） genotype of HPV in the 84 cases, the gene quantity are between 3.6 × 10^3 copies and 5.6 × 10^7 copies per milliliter. However there are 3 cervical cancer cases and CIN Ⅰ - Ⅲ 5 cases in the 13 HPV 16,18genotype cases. Conclusion：The results showed that the cervical cancer had high relationship with 16 and 18 genotype of the human papilloma vires.