神经生长因子对脊髓减压病神经损伤的修复作用研究

Protective effect of nerve growth factor on neuron injury in rat spinal cord decompression sickness

目的:研究神经生长因子对脊髓减压病神经损伤的修复作用。方法:SD大鼠随机分为正常对照组、安全减压组、生理盐水(NS)组、神经生长因子(NGF)治疗组、高压氧(HBO)暴露组、NGF与HBO联用组,建立大鼠脊髓减压病模型,采用酶联免疫实验(ELISA)定量检测比较各组大鼠出舱6、24、487、2 h肿瘤坏死因子(TNF-α)的蛋白表达。结果:NGF治疗组TNF-α浓度在6、24、48、72 h均明显低于生理盐水组(P<0.05),HBO治疗组TNF-α浓度在24、487、2 h也明显低于生理盐水组(P<0.05)。NGF与HBO联用组TNF-α的浓度在24、48、72 h均显著低于单纯NGF治疗组和HBO暴露组(P<0.05)。结论:神经生长因子和高压氧治疗可以抑制脊髓减压病大鼠TNF-α的蛋白表达,NGF与HBO联合治疗的效果明显优于单纯NGF治疗或HBO暴露。

Objective： To study the protective effect of nezve growth factor （NGF） on neuron injury in rat spinal cord decompression sickness. Methods： Rats were randomly divided into 6 group6： the control group; the safe deccmpression group; the normal saline （NS） group; the NGF group; the HBO group; and the NGF plus HBO therapeutic group. Then, an animal model of spinal cord decompression sickness was developed, and the expression of TNF-α （tumor necrosis factor-α） in each animal group was meatarot by ELISA at surface time, 6, 24, 48 and 72 h respeetivdy. Results： TNF-α of the NGF group is obviously lower than that of theNSgroup at 6, 24, 48 and 72 h （P〈0.05）. Thesameis true for the HBO Group at 24, 48 and 72 h （P〈0.05）. TNF-α in the NGF plus HBO therapeutic group is also signifieanfly lower than than of the NGF group or the HBO group at 24, 48 and 72 h （P〈0.05）. Conclusion： Both NGF and HBO can inhibit the expression of TNF-α in rats with spinal cord decompression sickness （P〈0.05）. NGF treatment plus HBO therapy can produce better effect than that of simple NGF treatment or HBO exposure （P〈 0.05）.