定量蛋白质组学中的同位素标记技术

Stable Isotope-coded Technology for Quantitative Proteomic Analysis

定量蛋白质组学的目的是对复杂的混合体系中所有的蛋白质进行鉴定,并对蛋白质的量及量的变化进行准确的测定,是当前系统生物科学研究的重要内容。近年来,由于质谱技术和生物信息学的进步,定量蛋白质组学在分析蛋白质组或亚蛋白质组方面已取得了令人瞩目的成就,但其最显著的成就应该归功于稳定同位素标记技术的应用。该技术使用针对某一类蛋白具有特异性的化学探针来标记目的蛋白质或肽段,同时化学探针要求含有用以精确定量的稳定同位素信号。在此基础上,实现了对表达的蛋白质差异和翻译后修饰的蛋白质差异进行精确定量分析。综述了在定量蛋白质组学中使用的各种同位素标记技术及其应用。

Quantitative proteomics, defined as the systematic identification of the proteins in complex samples and the determination of their quantity or quantitative change, is an important component of the emerging science of systems biology. Although advances in mass spectrometry and bioinformatics have contributed to quantitative proteome analysis, the highest precision of quantitation is based upon improved methods for chemichal and metabolic stable isotope labeling of proteins and peptides. Such projects typically combine the use of chemical probes that are specific for a targeted group of proteins and contain stable isotope signatures for accurate quantification with automated tandem mass spectrometry and bioinformatics tools for data analysis. The ability to quantify differences in protein expression and post-translational modifications using stable isotope labeling has been achieved. Technical and conceptual advances in quantitative subproteome profiling based on stable isotope- coded technology were summarized.