大鼠坐骨神经钳伤后变性和再生过程中S—100蛋白的变化

THE CHANGS OF S-100 PROTEIN DURING DEGENERATION AND REGENERATION OF RATS SCIATIC NERVES AFTER BEING CRUSHED

选用Wistar雌性大鼠40只,分设正常、神经损伤(钳伤右侧坐骨神经干)、损伤对照组.运用免疫组化PAP方法对神经于内的S—100蛋白检测.染色结果经形态学观察及电子计算机图像处理系统分析结果表明:坐骨神经干内S—100蛋白含量与损伤后神经再生时间有密切关系,并观察到损伤后第5、7天,损伤神经侧S—100轴浆转运现象,推测S—100可能参于了周围神经系统再生微环境的变化,从而影响损伤神经元的存活和神经纤维的再生.

The changes of S-100 protein during degeneration and regeneration were observed in 40 adult female wistar rats, which were intracardically perfused with 4% paraformaldehyde in 0. 1 phosphate buffer 1,3,5,7, 10, 14,21 days after the right sciatic nerve truck was crushed. The materials were taken from the crushed site to the proximal and distal part, and sectioned by freez-ing. The changes of S-100 protein were observed by immunohistochemistry PAP method and the findings were treated by computerized image analysis. The results showed that S-100 was closely related with nerve regeneration since its changes might affect the microenvironment of the peripheral nerve system.