豚鼠面神经损伤后运动终板及面神经核AchE含量的变化

Changes of AchE activity in motor end plate and facial nucleus after facial nerve injury of guinea pig

目的：研究面神经挤压损伤后运动终板（ＭＥＰ）、面神经核（ＦＮ）乙酰胆碱酯酶（ＡｃｈＥ）含量变化。方法：利用组织化学染色和图象分析技术，对ＭＥＰ和ＦＮ的ＡｃｈＥ进行量化分析。结果：正常口轮匝肌ＡｃｈＥ染色的ＭＥＰ在光镜下呈葡萄状或斑块状的椭圆形结构，锯齿状分布．面神经损伤４２ｄ，ＭＥＰ的数量及ＡｃｈＥ含量与正常对照组无明显差别．正常面神经核ＡｃｈＥ阳性神经元较大，部分胞体有突起。面神经核ＡｃｈＥ含量于面神经损伤后３ｄ升高，７ｄ达视峰，４２ｄ恢复正常．结论：面神经损伤后面神经核ＡｃｈＥ活性增高，这一变化可能有利于面神经损伤后的再生。

Objective: The acetylcholinesterase (AchE) contents were studied in the motor end plate (MEP) and facial nucleus (FN) after compression of facial nerve in guinea pigs. Methods: The AchE contents in MEP and FN were analysed by using histochemical staining and image analysis technique. Results: The MEP of normal musculus orbicalaris oris presented botryoid or psoriasis oval structure in shape and serraed distribution under light microscope. 42 d after injury, there were no significant changes of MEP counts and AchE contents, compared with normal group. The AchE positive neurons in normal FN were a little bit big, with processes on some cell bodies. It was noted that AchE contents increased on 3 d after injury, and continued to rise till the maximal level on 7 d, then gradually decreased and reached normal level after 42 d. Conclusion: AchE activity increased in facial nucleus after facial nerve injury, which might contribute to facial nerve regeneration.