IL-1β和TNF-α在小鼠巨细胞病毒性心肌炎组织中的表达及其意义

Expression of IL-1β and TNF-α in the myocardiumof MCMV myocarditis and its significance

目的研究IL-1β和TNF-α在巨细胞病毒(M CM V)心肌炎小鼠心肌组织中的表达及在心肌损伤中的作用。方法将60只4周龄BALB/C小鼠随机分为两组,实验组(36只)腹腔注射10-4TC ID M CM V,对照组(24只)注射3T 3细胞裂解液。观察腹腔注射后3、7、14d心肌组织中IL-1βTNF-α的表达及心肌病理改变。结果实验组心肌组织出现灶性或弥散性炎性细胞浸润、心肌细胞变性和坏死,对照组无病理改变。实验组IL-1β、TNF-α蛋白主要表达在炎性浸润细胞、变性或坏死的心肌细胞,而对照组几乎无表达。结论IL-1β、TNF-α在M CM V心肌炎心肌组织中表达,并可能在其发病中起重要作用。

Objective： To study the expression of IL-1β and TNF-α in the myocardium of murine cytomegalovirus （MCMV） myocarditis and to evaluate its effect on myocardial damage of MCMV myocarditis. Methods： Sixty BALB/C mice of 4 weeks were randomly divided into two groups： 36 were injected intraperitoneally with MCMV （experimental group） and 24 served as control group. Immunohistochemistry technique was used to detect IL-β and TNF-α expression in myocardium, and myocardial lesions were observed by histopathology. Results： Focal or diffuse lesions characterized by inflammatory cells and degeneration or necrosis of myocytes in experimental group were revealed in the myocardium; Positive staining signals of IL-1β and TNF-α proteins which mainly located in infiltrating inflammatory cells and degenerative or necrotic myocytes were markedly detectable. There were no positive findings in control group. Conclusion： IL-1β and TNF-α can be found in myocardium of MCMV myocarditis model. IL-1β and TNF-α may play important roles in pathogenesis of viral myocarditis.