ATP─MgCl2复合液抑制烫伤大鼠氧自由基和肿瘤坏死因子的生成

ATP MgCl 2 inhibits production of oxygen free radicals and tumor necrosis factor of burnt rat

目的：研究ＡＴＰ－ＭｇＣｌ２对烧伤早期自由基及肿瘤坏死因子（ＴＮＦ）的生成所产生的影响．方法：通过大鼠３０％总体表面积（ＴＢＳＡ）三度烫伤模型观察经ＡＴＰ－ＭｇＣｌ２治疗后，血清ＴＮＦ－α生物学活性及全血细胞化学发光峰时和峰值的变化．结果：烫伤６ｈ后，血清ＴＮＦ－α生物学活性明显升高（Ｐ＜０．０５或Ｐ＜０．０１），至伤后２４ｈ均维持在较高水平．伤后立即经ＡＴＰ－ＭｇＣｌ２治疗可明显抑制血清ＴＮＦ－α活性升高．伤后６～２４ｈ，中性粒细胞（ＰＭＮ）产生氧自由基的能力明显增强（Ｐ＜０．０５或Ｐ＜０．０１），但血浆调理活性降低．伤后立即给予ＡＴＰ－ＭｇＣｌ２治疗，可抑制ＰＭＮ产生氧自由基的能力，同时提高血浆对ＰＭＮ的调理作用．结论：ＡＴＰ－ＭｇＣｌ２可降低烧伤早期炎症介质ＴＮＦ的生物学活性，抑制ＰＭＮ过度产生自由基。

Objective: To investigate the influence of ATP MgCl 2 on serum tumor necrosis factor (TNF) and oxygen free radical (OFR) in the early stage after burn. Methods: A severely injured rat model of 30% total body surface area (TBSA) with full thickness skin scald was chosen for observation of the changes of biological activity of serum TNF α, the peak value and peak time of whole blood chemiluminescence of the rats treated with or without ATP MgCl 2. Results: Firstly, serum TNF α biological activity of the scald rats rose significantly from 6 h after burn and maintained at high level till 24 h, whereas such a trend was inhibited by the treatment of ATP MgCl 2 ( P <0.05 or P <0.01). Secondly, from 6 to 24 h after burn, the ability of polymorphonuclear neutrophil (PMN) to produce OFR increased significantly ( P <0.05 or P <0.01), while ATP MgCl 2 inhibited the OFR production of PMN. Conclusion: ATP MgCl 2, given to rats just after injury, can be beneficial for maintaining functions of important internal organs of severely burnt body by inhibiting the biological activity of TNF and decreasing the production of OFR of PMN.