HPLC法测定枸橼酸莫沙比利片的含量

Determination of Mosapride Citrate by HPLC

目的 建立测定枸橼酸莫沙比利片含量的HPLC方法。方法采用ODS色谱柱,磷酸二氢钾、庚烷磺酸钠溶液(取 磷酸二氢钾2．72g与庚烷磺酸钠1．0g加水溶解并稀释成1000ml)-乙腈(50:50)为流动相,检测波长为274nm。结果枸橼 酸莫沙比利在5μg·ml-1～50μg·ml-1范围内峰面积与浓度呈良好线性关系(r=0．9998),平均回收率为98．8％(RSD= 1．3％)n=6。结论 HPLC法测定枸橼酸莫沙比利含量,方法准确,重复性好,回收率高。

Objective The content of Mosapride Citrate was determined by HPLC.Methods ODS colunm was used, （KH2PO42.72g-Sodium heptanesulfonate 1.0g,dissolved and diluted with H20 to 1000ml） -Acetonitrile（50：50）as mobile phase, with detective wavelength at 274nm. Results The linear range was 5μg·ml^-1 - 50μg·ml^-1 （ r = 0. 9998）. The average recovery rate was 98.8 %, RSD was 1.3 % （ n = 6）. Conclusion The method was accurate, had a good reproducibility and high recovery.