排卵后小鼠卵周颗粒细胞在输卵管内死亡过程的研究

Study on Granulocytes' Death Processes in the Mouse Oviduct Ampulla after Ovulation

目的研究随同卵细胞排到输卵管内的卵周颗粒细胞的死亡过程。方法用组织学、TUNEL、免疫组织化学和透射电镜技术,对交配后5、81、2 h小鼠的输卵管进行观察。结果交配后5 h,输卵管壶腹部可见颗粒细胞围绕在卵细胞周围,但在12 h基本消失。5 h 5%颗粒细胞呈TUNEL阳性反应,8 h 30%呈阳性反应。超微结构研究显示,这些细胞以细胞核呈现常染色质凝集和向细胞核边缘聚集改变为特点。此外,一些颗粒细胞以线粒体的肿胀,细胞膜、核膜和其他细胞器的膜破裂为特点。在颗粒细胞间还可见F4/80阳性细胞,其胞质中有TUNEL阳性小体。结论随同卵细胞排到输卵管的颗粒细胞消失速度快,主要通过细胞凋亡和坏死2种细胞死亡途径,巨噬细胞可能参与凋亡细胞的清除过程。

Objective To investigate the death process of the granulocytes in oviduct after ovulation. Methods Oviducts were observed at 5, 8 and 12 h after mating with histology, TUNEL staining, immunohistochemistry, and transmission electron microscopy. Results All oviduct ampullae at 5 h after mating contained oocytes surrounded with numerous granulocytes, and the majority of granulocytes disappeared from the ampulle at 12 h. At 5 h after mating, approximately 5 % of granulocytes were TUNEL staining positive and, at 8 h, 30% were TUNEL staining positive. Ultrastructurelly, there were nuclear changes characterized mainly by compaction and margination of heterochromatin. These were designated as signs of classical apoptosis. In addition, some granulocytes, approximately 2% at 5 h and 13% at 8 h after mating, showed mitochondrial swelling and disruption of cellular, nuclear and the other organelle membranes. These were designated as typical signs of necrotic cell death. Among the granulocytes, there were F4/80 positive cells that contained TUNEL staining positive bodies. Conclusion Granulocytes died and disappeared rapidly from oviduct ampullas through two different death processes after ovulation, apoptosis and necrosis, and macrophages may be involved in apoptotic cell disposal pathways.