深海热液喷口周围微氧耐压细菌的培养研究

The Culture of micro-oxygen demanding and baroduric bacteria from a deep-sea hydrothermal vent

研究采用析因实验设计,探讨了培养基的水源、培养温度和生长pH等因素对喷口细菌生长繁殖的影响,初步探索了常压下东太平洋某海底喷口周围微氧耐压细菌的生长条件。样品培养10d后,对培养液中细菌量进行显微计数,实验数据用SPSS11.0统计软件中的方差分析程序进行处理。统计结果显示:在标准大气压下,从研究样品中获得最多细菌数的较优培养条件为:培养基水源为单蒸水;生长pH为7.60;培养温度为50℃。在该条件的培养液中细菌平均浓度达2.596×10个/mL。研究结果为进一步认识、鉴定与开发利用深海喷口周围细菌资源打下了基础。

To explore the growth condition of the micro-oxygen demanding and baroduric bacteria around a deep-sea hydrothermal vent at normal atmospheric pressure, factorial experimental design was used in the experiments. The influence of factors such as water sources, culture temperature, growth pH on growth and multiplication of the micro-oxygen demanding and baroduric bacteria around a deep-sea hydrothermal vent were investigated. After has been continuously cultured for ten days, the bacteria density in the culture fluid was estimated by counting and analyzing. Then the experimental data were processed by variance analysis using the variance procedure of SPSS 11.0. The results showed that at the standard atmospheric pressure, the highest density of bacteria can be obtained under the following conditions： distilled water, pH 7.60 and 50℃. Under the above conditions, the average density of the bacteria in the culture fluid was up to 2.596×10^6/mL. The results may devote to further cognition, identification, exploration and application of bacteria resources around hydrothermal vents.