摘要
为研究大肠杆菌不耐热肠毒素B亚单位(LTB)的佐剂活性。从大肠杆菌中调出LTB的原始基因,将该基因克隆、构建pET21b-LTB表达载体、转化大肠杆菌BL21(DE3)进行表达,并对表达产物进行初步纯化;经DNA测序、SDS-PAGE、ELISA检测,结果表明成功构建了能够稳定表达可溶性LTB的菌株,并获得初步纯化LTB的方法。为今后LTB的研究及应用奠定了基础。
In order to achieve the expression of LTB in E. coli BL21（DE3） and to study its adjuvant activity. LTB gene was amplitied by PCR, and pET- 21b- LTB expression vector was constructed, then it was transformed in E. coli BL21 （DE3）. It was detected by sequencing DNA, SDS -PAGE and ELISA. Result： the expression of LTB was constructed successfully, the way for purify LTB was obtain.
出处
《生物技术》
CAS
CSCD
2005年第6期17-20,共4页
Biotechnology
基金
甘肃省自然科学基金(No.3ZS051-A25-094)
关键词
大肠杆菌不耐热肠毒素B亚单位
表达
纯化
粘膜佐剂
Escherichia coli heat -iabile enterotoxin B subuint
expression
purification
mucosal immunoadjuvant
