逆转录—聚合酶链式反应技术检测“歇马杏”主要病毒

Detection of Main Viruses in Xiema Apricot by RT-PCR

目的:调查“歇马杏”携带主要病毒的情况。方法:利用作者已建立的ACLSV、PNRSV和PDV RT-PCR检测体系对辽宁省大连市地方特产“歇马杏”田间植株携带三种病毒的情况进行了调查。结果:被检植株ACLSV、PNRSV和PDV的单独感染率分别为83.33%、83.33%和90%,复合感染率达到76.67%,但带毒程度不同。

The RT- PCR detection systems for ACLSV,PNRSV and PDV were used to investigate whether Xiema apricot in orchards was infected by the three kind of virus or not. The result showed that all the trees were infected singly or complexly by ACLSV, PNRSV and PDV, but the in- fected degree was different.