东莞地区儿童肺炎链球菌携带率及耐药性检测

Examination of antibiotic resistance of streptococcus pneumoniae isolated from children in Dongguan City

目的分析东莞地区学龄前健康儿童鼻咽部携带肺炎链球菌情况及对常用抗生素的耐药特点,为预防儿童肺炎链球菌感染及合理使用抗生素提供参考。方法采用专用巧克力平板和血平板培养基,对采集的350例3～5岁儿童鼻咽部分泌物作培养鉴定,对鉴定明确的肺炎链球菌根据美国临床实验室标准化委员会(NCCLS)标准进行常用抗生素检测。结果东莞地区儿童肺炎链球菌携带率约为34.5%;携带株对左旋氧氟沙星、氯霉素、复方新诺明、苯唑西林、四环素、红霉素和青霉素的耐药率分别是2%、24%、42%、64%、74%、78%和24%,仅对万古霉素敏感。结论东莞地区健康学龄前儿童鼻咽部肺炎链球菌携带率高于国内许多地区,对常用抗生素耐药性严重,并存在多重耐药现象。

Objective To analyze streptococcus pneumoniae（SP） isolated from healthy children in Dongguan City and their antibiotic resistance. Methods 350 samples of rhinopharyngeal secretion from children aged 3 to 5 years old were cultured with special chocolate and blood medium. SP and Hi isolated were tested for antibiotic susceptibility according to NCCLS. Results The detection rate of SP was 34.5%. The resistance rates of SP to LEV, C, SXT, OX, TE, E and PG were 2%, 24%, 42%, 64 %,74 %,78% and 24 % respectively. Conclusion The detection rate of Sp in children in Dongguan City is higher than in other regions. And the situation of resistance to antibiotic and multi-resistant is serious.