CON43、CON26蛋白在子宫内膜异位症中的表达及意义

Expression of Connexin 43 and Connexin 26 Protein and its Significance in the Patients with Endometriosis

目的:探讨间隙连接蛋白(CON)43、CON26在子宫内膜异位症中的表达及意义。方法:对20例子宫内膜异位症(内异症)内膜用免疫组化法检测CON43和CON26蛋白的表达,并与21例其他良性疾病子宫的内膜进行比较。结果:CON43蛋白主要在内膜腺上皮细胞膜和细胞浆中表达,而CON26阳性染色出现在子宫内膜腺上皮细胞和基质细胞的细胞浆中,而在细胞核中无阳性染色。内异症组中CON43和CON26蛋白表达均较对照组显著降低。结论:由CON43和CON26蛋白介导的子宫内膜各种细胞间的间隙连接,在细胞间的物质和信号的传递中起重要作用。子宫内膜异位症中CON43和CON26蛋白表达的降低,可能导致腺上皮间、基质细胞间以及腺上皮与基质细胞间的物质和信号传递减低或缺失,可能在内膜细胞的游走、粘附于腹膜中起一定作用。

Objective： To study the expression of Connexin 43 and Connexin 26 protein and its significance in patients with endometriosis. Methods： The 20 endometrlal samples of endometriosis （study group） and 21 endometri um of other gynecological disease （control group） were obtained by hysterectomy. Immunohistochemical staining with antibody against Connexin 43 and Connexin 26 was performed by S - ABC method. Results. Connexin 43 protein mainly expressed in the membrane and plasma of glandular epithelium of endometrium. But Connexin 26 protein not only expressed in the plasma of glandular epithelium, but also expressed in the plasma of stromal cell, but lack of nuclei stainings. The expression of Connexin 43 and Connexin 26 protein in endometriosis was much lower than the control group. Conclusions： The gap junction induced by Connexin 43 and Connexin 26 protein between the glandular epithelial cells, stromal cells, and glandular epithelial cell and stromal cell of endometrium is very important for transduction of substances and signals. The loss of gap junction communication caused by the reduction of Connexin 43 and Connexin 26 protein in endometriosis might be responsible for the travel of endometrial cell to other places.