中国青海地区喜马拉雅旱獭嗜肝病毒自然感染的组织学研究

Natural Infection of Hepadnavirus in Liver Tissues of Marmota himalayana in Qinghai,China

应用原位杂交技术、免疫组化技术以土拨鼠肝炎病毒(Woodchuck hepatitis virus,WHV)的检测系统检测50份 喜马拉雅旱獭肝组织可能存在的嗜肝病毒c基因、s抗原及c抗原的表达,同时检测肝脏组织病理学改变。结果显示 旱獭肝组织中嗜肝病毒s抗原、c抗原的阳性率分别为26％(13／50)、36％(18／50);在抗原双阳性的10份肝组织标本 中有c基因的阳性表达,阳性率为50％。c抗原定位于肝细胞胞浆和／或胞核,呈散在、片簇状分布,c基因定位于肝细 胞的细胞核,阳性细胞散在分布。50份标本中5份出现肝炎的病理改变,与抗原检出间无明显相关性。使用WHV 的病毒检测系统证实青海地区喜马拉雅旱獭可能存在类似WHV的嗜肝病毒感染,从组织学的角度为中国青海地区 喜马拉雅旱獭嗜肝病毒自然感染提供证据,此种动物有可能用于建立嗜肝病毒感染的动物模型。

Hepadnavirus surface, core antigen and c genes and their products which may exist in liver tissues of Marmots himalayana were detected by immunohistochemical staining and in situ hybridization, respectively. The data suggested that hepadnavirus surface antigens were detected in the liver of 13 out of 50 animals （26%） and the core antigen in 18 out of 50 animals （36%）. Some of the samples were proven positive by derection of the c gene by in situ hybridization. The core antigen was located in the cytoplasm and/or nucleus of liver cells and had multiple appearance such as scatterd, clustered or diffused. The C antigen was located in a scattered manner in the nucleus of liver cells. Mild inflammation was observes in 5 liver sections and did not correlate to antigen expression. Using histological detection methods of Woodchuck hepatitis virus （WHV）, we demonstrated that a hepadnavirus similar to WHV infection may exist in Marmota himalayana in Qinhai, China. Marmota himalayana may be a potential animal model to study hepadnavirus infection.