多溴联苯醚对大鼠肝细胞CYP1A2依赖性MROD活性的影响被引量：8

MROD Analysis of Competitive Inhibition from Polybrominated Diphenyl Ethers to CYP 1A2 in Rat Hepatocytic Microsomes

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摘要采用未成熟SD大鼠肝细胞微粒体为试验材料,以2,3,7,8-四氯-二苯基-并-二哑噁英(2,3,7,8- tetrachlorobenzo-p-dioxin,TCDD)作为参照,利用甲氧基-异酚噁脱甲基酶(methoxyresorufin-O- demethylase,MROD)竞争性抑制动力学和荧光光谱法分析法,测定了5种多溴联苯(polybrominated diphenyl ethers,PBDEs)对7-甲氧基-异酚噁唑脱甲基反应的影响,间接研究了这些PBDEs对CYP1A2 的竞争性抑制作用。结果表明,和TCDD相比,PBDEs对MROD活性的抑制作用较弱,亦即对CYP1A2的竞争性抑制作用较弱。 The role of a number of polybrominated diphenyl ethers （PBDEs） as inhibitors of the demethylation of 7-methoxyresorufin, a process that is selectively catalyzed by cytochrome P450 1A2 （CYP1A2）, was examined, using methoxyresorufin-O-demethylase （MROD） fluorescent assay and competitive inhibition constant Ki determination. It was found that those examined PBDEs showed punier inhibiting effects to MROD / CYPtA2 than that of 2,3,7,8-Tetrachlorobenzo-p-dioxin （TCDD） which is the strongest inhibitor of MROD / CYP1A2 activity in dioxin-like compounds.

作者聂芳红陈进军 Bunce Nigel

机构地区广东海洋大学食品科技学院广东海洋大学动物医学系 Dept.of Chemistry and Biochemistry

出处《中国农学通报》 CSCD 2005年第12期11-13,共3页 Chinese Agricultural Science Bulletin

基金教育部留学回国人员科研启动基金(教外司[2003]406号)广东海洋大学SCI源刊作者奖励基金(0412130)

关键词 MROD分析法 CYP1A2 多溴联苯醚大鼠 MROD assay, CYP 1A2, Polybrominated diphenyl ethers, Rat

分类号 TQ241.3 [化学工程—有机化工] Q347 [生物学—遗传学]

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