等位特异PCR方法检测IL-6基因启动子区-634C/G多态性

Allele Specific PCR Method Detecting the Polymorphism of IL-6 Gene Promoter Region-634C/G

目的:应用等位特异PCR法检测IL-6启动子区-634位C/G变异,以探讨ASPCR应用的可行性.方法:应用ASPCR法和PCR-RFLP法同时检测101例研究对象IL-6启动子区-634位C/G变异的多态性,并对二者的结果进行比较研究.结果:(1)除一个CC基因型结果不一致外,其余的CG、GG和CC基因型两种方法的结果都一样;(2)ASPCR在单点突变多态性的检测中,具有省时、快速和成本低等优点.结论:APCR方法检测基因的已知突变,具有快速、准确、省时、价格低等优点,在满足引物模板间碱基错配能阻止引物延伸的条件下,可优先选用此法.

Objective： To detect the polymorphism of IL- 6 gene promoter region - 634 C/G by allele specific PCR method. Methods： The polymorphism of IL - 6 gene promoter region - 634 C/G in 101 subjects whose genotypes were detected by PCR- RFLP were detected by allele specific PCR method, the results of the two methods were tested and verified with each other, and the two methods were assessed. Results： （1） The results of CG, GG and CC genotypes were identical except one case of CC genotype in the two methods. （2） Allele specific PCR method was speedy and accurate with a low cost. Conclusion： When detecting a known point mutation of certain gene, allele specific PCR method will be given priority over PCR - RFLP method if mismatching of base and primers could prevent primer extension.