Calpain10基因多态性与超重肥胖、胰岛素抵抗的相关性研究

A Study on Association of Calpain 10 Gene Polymorphism with Overweight,Obesity and Insulin Resistance

目的:探讨昆明地区2型糖尿病(T2DM)钙蛋白酶10基因(Calpain10基因)的多态性(SNP)与超重肥胖、胰岛素抵抗(IR)的关系.方法:选取昆明市城市社区居民非超重肥胖者111名及超重肥胖者151名.检测空腹血糖(FPG)、空腹胰岛素(FINS)、C肽(C-P)水平,计算胰岛素抵抗指数(HOMA-IR).应用聚合酶链式反应双引物等位基因特异性扩增法(PCR-ASA)检测Calpain10基因SNP43位点(G/A)及SNP44位点(T/C)基因型.结果:(1)GG基因型频率随着BMI值的增大呈逐渐增加的趋势,但各组基因型频率间无显著性差异(P>0.05);(2)BMI>28.00组的TC基因型频率显著高于其余各组(P<0.05);(3)GG基因型组中,WHR≥0.90者的FPG、FINS、C-P水平及HOMA-IR均显著高于WHR<0.90者(P<0.05);(4)TT基因型组中,WHR≥0.90者的FPG、FINS、C-P水平及HOMA-IR均显著高于WHR<0.90者(P<0.05).结论:昆明地区人群中Calpain10基因SNP44的多态性与肥胖有关,同为TT基因型者中具有中心性肥胖者存在明显的胰岛素抵抗现象.SNP43的多态性与肥胖无关,但同为SNP43GG基因型者中具有中心性肥胖者存在明显的胰岛素抵抗现象.

Objective： To probe the association of single nucleotide polymorphism of Calpain 10 gene with insulin resistance, overweight and obesity in Kunming districts. Methods： 111 subjects characterized as non-overweight and obesity and 151 subjects characterized as overweight and obesity were studied in Kunming urban communities. Fasting plasma glucose, insulin, C- peptide were analyzed. The genotype of site SNP43 （G/A） and SNP44 （T/C） in Calpain 10 gene was detected by Polymerase chain reaction and Allele specific amplification （PCR- ASA）. Results： （1） With the increase of BMI, there was a tendency of increase in the frequency of “GG” allele of the four BMI groups, but there was no significant difference among the four BMI groups （P〉0.05） ; （2） Frequency of “TC” allele of the subjects with BMI〉28.00 was significantly higher than that of the other groups （ P〈0.05）; （3） FPG, FINS, C-P and HOMA- IR of the subjects with WHR≥0.90 was higher than that of the subjects with WHR〈 0.90 among the subjects with “GG” Genotype （P〈0.05）; （4） FPG, FINS, C-P and HOMA- IR of the subjects with WHR≥ 0.90 were higher than those of the subjects with WHR 〈0.90 among the subjects with “TT” Genotype （/9 〈0.05）. Conclusion： Polymorphism of site SNP44 of Calpain 10 gene was related with obesity. There exited insulin resistance among the subjects who were characterized as central obesity with SNP44 “TT” Genotype. Polymorphism of site SNP43 was not related with obesity, but there exited insulin resistance among the subjects who were characterized as central obesity with SNP43 “GG” Genotype in kunming districts.