以口腔粘膜细胞为对照检测异基因造血干细胞移植后嵌合状态

Chimerism Testing after Allogeneic Peripheral Blood Stem Cell Transplantation(allo-PBSCT) with Oral Muomembranous Cells as Contrast Sample

目的:探讨以口腔粘膜细胞DNA代表移植前基因状态的可行性,解决缺乏移植前样本病例嵌合状态的检测问题.方法:对2例接受异基因造血干细胞移植术病例,采集供者、受者移植术前外周血、受者移植术后外周血和口腔粘膜拭子,以PCR-STR技术对各样本进行DNA分析,对比移植前后基因分型变化判断嵌合状态,比较受者移植术前外周血和移植术后口腔粘膜拭子基因分型的异同.结果:2病例移植后受者外周血的PCR-STR基因分型与供者一致,呈完全的供者嵌合状态;受者移植后的口腔粘膜细胞基因分型与移植前外周血的基因分型一致.结论:口腔粘膜细胞的DNA样本可以代表受者移植前的基因状态,在实践中可以用口腔粘膜细胞代替移植前的受者样本进行嵌合状态的检测.

Objective： To study the feasibility of using oral muomembranous cells as contrast sample for chimerism testing after allogeneic peripheral blood stem cell transplantation （allo - PBSCT） when the contrast sample before operation isn＇t available. Methods： 2 sample.s, one from peripheral blood of donor, the other from that of acceptor before allo - PBSCT and the peripheral blood and oral muomembranous cells from acceptor after allo - PBSCT were collected , the DNA was extracted and analyzed by PCR - STR technique. Results： The genotype of the acceptor＇s peripheral blood before allo - PBSCT and oral muomembranous cells after allo- PBSCT was identical. The genotype of peripheral blood from the acceptor after allo - PBSCT and that of peripheral blood from the donor was identical. Conclusion： The genotype of oral muomembranous cells after allo-PBSCT may stand for the samples before operation, thus it can be used as contrast sample for chimerism testing, especially when the contrast sample before operation isn＇t available.