摘要
对农用抗生素2-16产生菌(Streptomyces ahygroscopicusvar.huangshanensis)依次进行紫外线诱变、NTG诱变、低能碳离子注入处理,筛选获得高产菌株515号,效价较出发菌株0号提高223.10%。利用SAS软件提供的P lackett-Burm an设计和响应面分析法对菌株515号的发酵培养基进行优化,采用优化培养基后效价较原始培养基提高38.53%。
In order to improve the production of agro-antibiotic 2-16, the producing strain （Streptomyces ahygroscopieus var. huangshanensis ） was treated by protoplast regeneration, ultraviolet radiation, NTG mutagenesis and low energy C^＋ ion implantation. At last, a high-yield strain No. 515 was obtained. The production of No. 515 was increased by 223.10%. By using Plackett-Burman design and Response Surface Analysis provided by SAS software, the cultivation condition of No. 515 was optimized. The amount of agro-antibiotic 2-16 was increased by 38.53% when the strain No. 515 was cultivated in the optimum medium instead of the initial one.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第6期7-11,共5页
Microbiology China
基金
十五科技攻关资助项目(No.2001BA509B0602)
