水体病毒浓缩方法的建立和优化研究

Studies on Establishing and Optimizing Conditions of Concentration Virus in Water Body

采用氯化钙(CaC l2)、聚乙二醇(PEG,pH7.0)、聚乙二醇(PEG,pH11.5)、三氯化铝(A lC l3)沉淀、Am icon Utcra超滤离心装置和硝酸纤维素吸附膜6种浓缩方法,浓缩人工添加于水体的1型脊髓灰质炎疫苗病毒(PV1),并对浓缩实验条件进行选择和优化。结果表明,CaC l2和聚乙二醇(pH7.0)沉淀法适用于浓缩大容量水体中的病毒,而超滤离心管浓缩法适用于小容量水体,这3种浓缩方法的病毒回收率均达到100%。

In this experiment six methods, calcium chloride （CaCl2 ） precipitation, polyethylene glycol （PEG, pH7. 0） precipitation, polyethylene glycol （ PEG, pH11. 5 ） precipitation, aluminum chloride （ AlCl3 ） precipitation, Amicon Utcra centrifugal filter devices and cellulose nitrate membrane were used to concentrate the vaccine poliovirus type 1 （PV1） added to water body; experimental conditions for concentration were selected and optimized. The results showed that two methods, CaCl2 and PEG （pH 7. 0） precipitation were suitable for concentrating virus in large volumes of water while amicon utcra centrifugal filter devices for small ones. The virus recovery of the three methods reached a 100% rate.