摘要
通过正交设计试验,优化了青霉(Penicillium)属真菌SHZK-15产胞外布雷菲德菌素(B refeld in-A,简称BFA)的液体发酵条件。最佳的BFA发酵条件为:培养基含马铃薯200g(煮汁),葡萄糖20g,(NH4)2SO44.0g,KH2PO41.0g,MgSO4.7H2O 2.0g,CaCO35.0g,pH自然,瓶装量为100mL/250 mL,培养温度为28℃,转速为120 r/m in,培养时间为7d,BFA的最高产量可达151.6mg/L。
Through orthogonal experiment, the optimal conditions were given for producing extracellular BFA of Penieillium. Its optimal conditions were as follows （g/L） ： potato 200, glucose 20, （NH4 ）2 SO4 4.0, KH2PO4 1.0, MgSO4·7H2O 2.0, CaCO3 5.0, the pH was natural, culture medium volume 100mL/250mL, culture temperature 28℃, revolutions 120r/min and culture time 7 days. The biggest production of BFA was 151.6 mg/L.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第6期52-57,共6页
Microbiology China
关键词
布雷菲德菌素
青霉
发酵
正交试验
Brefeldin-A , PeniciUium, Fermentation, Orthogonal experiment
