实时荧光PCR技术对小麦矮腥黑穗病菌的检测

Detection and Identification of Tilletia controversa Kühn by Real-time fluorescent PCR

通过对小麦矮腥黑穗病菌(TCK)及其近似种小麦网腥黑穗病菌(TCT)和小麦光腥黑穗病菌(TFL)的rDNA序列ETS区间测序比较分析,找出了TCK相对于TCT和TFL的特异性序列,并根据TCK的特异性序列设计了实时荧光PCR探针,利用实时荧光PCR技术成功实现了对TCK的检测。

By analysing the ribosome gene （rDNA） sequences in externally transcribed spacer （ETS） of wheat T. contraversa （TCK） and its similar spacies T. caries （TCT） and T.foetida （TFL） . The special sequences of TCK＇s ETS have been found, . And designed Taqman probe according to the special sequences, TCK has been successfully detected by using Real-time Fluorescent PCR.